So far in applied conditions and extracts endogenous S6K1/2 protein is detectable as a very weak band,Keeping the samples at 4°C all times is of crucial importance, Laucs buffer is used in example below since this is the one which is used routinely by this test laboratory for the work with kinases, Some unspecific bands can be seen, depending upon western blot protocol which is used, Therefore please consider to use a negative control together with your samples
Ribosome S6 kinase 1/2 (S6K1/2) is involved in TOR signaling and osmotic stress response. It is activated by PDK1 and inhibited by osmotic stress. It is expressed in all tissues, especially in the high metabolic activity of growing buds, root tips, leaf margins and germinated seeds. Alternative names :AtPK1/AtPK6 S6K1), AtPK2/AtPK19 (S6K2).
For research use only, not for clinical use.