S6K1/2 | Ribosomal S6 kinase 1/2
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S6K1/2 | Ribosomal S6 kinase 1/2

Cat: PA00842
Size: 50 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana
Datasheet:

Product Info

Immunogen
KLH-conjugated peptide, derived from Arabidopsis thaliana S6K1: UniProt: P42818, TAIR: AT3G08730 and S6K2: UniProt: Q39030, TAIR: AT3G08720. Due to high amino acid homology, chosen peptide is conserved in both proteins:  S6K1 and S6K2.
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4. Contains 0.02% sodium azide.
Format
Lyophilized
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 750-1 : 1000 (WB)
Expected | apparent MW
52,6 kDa (S6K1) and 53 kDa (S6K2)

Reactivity

Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Physcomitrella patens, Thelungiella halophila
Not reactive in
Setaria viridis, Solanum lycopersicum; Vitis vinifera

Additional information

So far in applied conditions and extracts endogenous S6K1/2 protein is detectable as a very weak band,Keeping the samples at 4°C all times is of crucial importance, Laucs buffer is used in example below since this is the one which is used routinely by this test laboratory for the work with kinases, Some unspecific bands can be seen, depending upon western blot protocol which is used, Therefore please consider to use a negative control together with your samples

Description

Ribosome S6 kinase 1/2 (S6K1/2) is involved in TOR signaling and osmotic stress response. It is activated by PDK1 and inhibited by osmotic stress. It is expressed in all tissues, especially in the high metabolic activity of growing buds, root tips, leaf margins and germinated seeds. Alternative names :AtPK1/AtPK6 S6K1), AtPK2/AtPK19 (S6K2).

For research use only, not for clinical use.