Rubisco | 557 kDa hexadecamer
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Rubisco | 557 kDa hexadecamer

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Cat: PA00479
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Anabaena sp. PCC 7120, Arabidopsis thaliana, Aucuba japonica, Fremyella diplosiphon, Glycine max, Hordeum vulgare, Manihot esculenta Crantz, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus sp., Salsola laricifolia, Solanum tuberosum, Spinacia oleracea, Synechocystis sp. PCC 6803, Synechococcus sp. PCC7942, Zea mays
Datasheet:

Product Info

Immunogen
purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000), UniProt: P00875 and Q43832
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunolocalization (IL), Western blot (WB)
Recommended dilution
1 : 10 000-1 : 20 000 on 0,5-10 ug total cellular protein/lane and standard (WB), 1 : 500-1:1000 (IL)
Expected | apparent MW
53-55 | 53-55 kDa

Reactivity

Confirmed reactivity
Anabaena sp. PCC 7120, Arabidopsis thaliana, Aucuba japonica, Fremyella diplosiphon, Glycine max, Hordeum vulgare, Manihot esculenta Crantz, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus sp., Salsola laricifolia, Solanum tuberosum, Spinacia oleracea, Synechocystis sp. PCC 6803, Synechococcus sp. PCC7942, Zea mays
Predicted reactivity
higher plants, algae, Nannochloropsis sp.
Not reactive in
Chlamydomonas reinhardtii (immulolocalization)

Additional information

RbcS subunit is not detected by this antibodyThis product can be sold containing proclin if requested

Description

Rubisco(ribulose-1, 5-diphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of carbon dioxide fixation in photosynthetic organisms. It shares a clear homology with purple bacteria and flowering plants and consists of two protein subunits, each with eight copies. In plants and green algae, the large subunit (~55 kDa) is encoded by the chloroplast rbcL gene, and the small subunit (15 kDa) is encoded by a family of nuclear red cell genes.

For research use only, not for clinical use.