RPT2a | Regulatory particle triple-A ATPase subunit 2a

Inquiry

Cat: PA01884

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

Recombinant, full lenght RPT2a of Arabidopsis thaliana RPT2a protein sequence UniProt: Q9SZD4-1, TAIR: At4g29040 overexpressed in E.coli, purified from a gel piece

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Storage

Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 3000 (WB)

Expected | apparent MW

49,4 | 50 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Brassica napus, Cajanus cajan, Gossypium hirsutum, Mucuna pruriens, Noccaea caerulescens, Vigna radiata var. radiata

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

This antibody is also recognizing RPT2b protein. Recommended western blot conditions: SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk.

Description

RPT2a (Regulatory particle triple-A ATPase subunit 2a) is a 26S proteasome subunit. It is necessary for the maintenance of root meristem and regulates gametophytogenesis. RPT2a has also been shown to regulate gene silencing through DNA methylation. Alternative names :26S proteasome AAA-ATPase subunit, RPT2a 26S proteasome subunit 4 homolog A, protein stop root, 26S proteasome regulatory subunit 4 homolog A.

For research use only, not for clinical use.