RPN5a | 26S proteasome regulatory subunit, putative (RPN5)

Inquiry

Cat: PA00677

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

Recombinant RPN5a of Arabidopsis thaliana UniProt:F4KFD7-1, TAIR: At5g09900, overexpressed in E.coli, purified from a gel piece

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Storage

Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 5000 (WB)

Expected | apparent MW

52,7 | 50 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Glycine soja, Gossypium arboreum, Juglans regia, Morus notabilis, Noccaea caerulescens, Theobroma cacao

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

This antibody is also recognizing RPN5b.Recommended protein load is 20 µg/well, primary antibody dilution: 1: 1000 ON/4°C and optimisation based on obtained result regarding signal/noise ratio.

Description

RPN5a | 26S proteasome regulatory subunit (RPN5) is one of two isomers of the 26S proteasome regulatory protein (RN) subunit RPN5. For many functions, it acts redundantly with the collateral gene RPN5b, but also appears to play an independent role.

For research use only, not for clinical use.