RPN10 | 26S proteasome regulatory subunit RPN10

Inquiry

Cat: PA01880

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

HIS-tagged RPN10 of Arabidopsis thaliana UniProt: P55034-1, TAIR: At4g38630 expressed in E. coli and purified by metal-chelate affinity chromatography

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Storage

Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 3000 (WB)

Expected | apparent MW

40,7 | 40 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Gossypium hirsutum, Noccaea caerulescens, Theobroma cacao

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

For western blot detection image, please check van Nocker et al. (1996).

Description

RPN10 (26S proteasome regulatory subunit RPN10) plays a role in maintaining the structural integrity of 26S proteasome subcomplex 19S regulatory granules (RP) by directly or indirectly recognizing ubiquitin /26S proteasoma-mediated proteolysis (UPP) as a ubiquitination substrate. Bind and may select ubiquitin couplings for destruction. Plays a role in growth and development through proteasoma-dependent ABA-signaling protein ABI5/DPBF1 degradation, as well as balancing cell expansion and cell proliferation rates during stem development. Alternative names :26S proteasome non-ATPase regulatory subunit 4 homology, AtRPN10, 26S proteasome regulatory subunit S5A homology, polyubiquitin chain binding protein 1,AtMCB1, MBP1.

For research use only, not for clinical use.