RNA-dependent RNA polymerase 6 (RDR6), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana RNA-dependent RNA polymerase 6 (RDR6) , partial

Product Gene Name

RDR6 recombinant protein

Product Synonym Gene Name

RDR6

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

MGSEGNMKKS VVTQVSIGGF GESTTAKQLT DYLEDEVGIV WRCRLKTSWT PPGSYPNFEI ADTSNIPSID _x000D_
EYKKVEPH AFVHFAVFES AGRAMDAAGQ CNLILDGQPL KVSLGPKNPY SLNQRRRTTV PY

Sequence Positions

1-130. partial

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

136,929 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

RNA-dependent RNA polymerase

NCBI Accession #

NP_001327617.1

NCBI GI #

1063715831

NCBI GenBank Nucleotide #

NM_001339423.1

NCBI GeneID

824112

NCBI Official Full Name

RNA-dependent RNA polymerase 6

NCBI Official Symbol

RDR6

NCBI Official Synonym Symbols

AtRDR6; RNA-dependent RNA polymerase 6; SDE1; SGS2; SILENCING DEFECTIVE 1; SUPPRESSOR OF GENE SILENCING 2

NCBI Protein Information

RNA-dependent RNA polymerase 6

NCBI Summary

Encodes RNA-dependent RNA polymerase. Involved in trans-acting siRNA and other siRNA biogenesis. Required for post-transcriptional gene silencing and natural virus resistance.

UniProt Gene Name

RDR6

UniProt Synonym Gene Names

RDRP6; SDE1; SGS2; AtRDRP6

UniProt Protein Name

RNA-dependent RNA polymerase 6

UniProt Synonym Protein Names

Protein SILENCING DEFECTIVE 1; Protein SUPPRESSOR OF GENE SILENCING 2; RNA-directed RNA polymerase 6

UniProt Primary Accession #

Q9SG02

UniProt Secondary Accession #

Q9LKP0; A0A1I9LSW2

UniProt Related Accession #

Q9SG02

UniProt Comments

RNA-dependent RNA polymerase involved in post-transcriptional gene silencing (PTGS). Possesses ssRNA and ssDNA-dependent polymerase activity, but does not have priming activity. Possesses in vitro 3' nucleotidyltransferase activity in the presence of UTP as single nucleotide. Required for the production of 21 nucleotide trans-acting small interfering RNAs (ta-siRNAs) derived from TAS1, TAS2 and TAS3 endogenous transcripts. Acts in the RDR6/SGS3/DCL4/AGO7 ta-siRNA pathway involved in leaf developmental timing. Required for the production of natural siRNAs (nat-siRNAs) derived from cis-natural antisense transcripts. Required for the production of 24 nucleotide nat-siRNAs derived from the stress-related P5CDH-SRO5 antisense gene pair. Required for PTGS induced by transgene direct repeats. Plays an essential role in transitive silencing of transgenes by processing secondary siRNAs. This pathway, which requires DCL2 and DCL4, amplifies silencing by using the target RNA as substrate to generate secondary siRNAs, providing an efficient mechanism for long-distance silencing. Involved in the biogenesis of secondary siRNAs which require 22 nucleotide miRNAs associated to AGO1. Participates synergistically with AS1 and AS2 to proper plant development by repressing the miR165 and miR166 microRNAs (independently of AGO10) that may lead to mRNA degradation of genes in the class III HD-ZIP family. Required for the production of some small RNAs derived from the crucifer-infecting tobamovirus (TMV-cg). Required for sense virus-induced post-transcriptional gene silencing (S-PTGS).