Ricinus Communis Agglutinin I (RCA I, RCA120), Rhodamine

Inquiry

Cat: PL00667

Size: 5 mg

Sugar Specificity: Galactose

Datasheet:

Description

This lectin consists of two 60 kDa subunits that can be dissociated by a reducing agent into a closely related chain between 27 kDa and 33 kDa. One of these chains appears to be common to the B chain of ricin, another castor agglutinin, while the other chain is specific to RCA i. The B chain binds to galactose or n-acetylgalactosamine residues of the membrane glycoconjugate. Rhodamine-labeled castor lectin I has the appropriate amount of fluorescent dye binding to provide the best staining properties for this lectin. This conjugated substance basically does not contain unconjugated fluorescent dyes. The excitation maximum is 550 nm and the emission maximum is 575 nm.

Product Info

Conjugate

Rhodamine

Concentration

5 mg active conjugate/ml

Sugar Specificity

Galactose

Storage

2-8℃

Excitation

545-555 nm

Emission

570-580 nm

Fluorescence

Red

Application

Immunofluorescence, Glycobiology

Recommended dilution

For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.

Solution

10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 5 mM lactose

For research use only, not for clinical use.