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Ricinus Communis Agglutinin I (RCA I, RCA120), Agarose bound
Cat: PL00662
Size: 2 ml/10 ml
Sugar Specificity: Galactose
Datasheet:
Description
Agarose bound castor lectin I is prepared using our affinity purified lectin. This lectin consists of two 60 kDa subunits that can be dissociated by a reducing agent into a closely related chain between 27 kDa and 33 kDa. One of these chains appears to be common to the B chain of ricin, another castor agglutinin, while the other chain is specific to RCA I.
Product Info
Conjugate
Agarose
Sugar Specificity
Galactose
Storage
2-8℃
Application
Glycobiology, Affinity Chromatography
Recommended dilution
Wash gel thoroughly with buffer before use to remove sugar added to stabilize the lectin. Recommended product for eluting glycoconjugates bound to this agarose-lectin: Glycoprotein Eluting Solution, Cat. No. ES-2100. Alternatively, 200 mM galactose or lactose can be used. After use, wash the gel with several column volumes of buffered saline, then resuspend gel in buffered saline containing 0.08% sodium azide for storage.
Solution
10 mM HEPES, pH 7.5, 0.15 M NaCl, 20 mM lactose, 0.08% sodium azide
For research use only, not for clinical use.