Ricin (RTA subunit), clone MMA

Antibodies have been purified by affinity chromatography on Protein G from tissue culture supernatant and lyophilized from solution containing: mannitol 1%, dextran 1%, NaCl 100mM, sodium phosphate 10 mM, pH 7.5. If required, mannitol can be removed either by dialysis or by chromatography using mini columns Sephadex G50 fine. The concentration can be measured by absorbance at 280 nm (1.35 for 1 mg/ml) or by Bradford method Ricin detection limit is 5 ng/ml, signal-to-noise ratio is 15-50 up to 100 ng/mlEpitope recognized by this antibody is located on the surface of RTA subunit and not accessible in intact ricin molecule.  Suggested protocol can be found here.,Detected epitope is located on the surface of RTA subunit not accssible in intact ricin molecule, This antibody does not cross-react to whole ricin molecule

Ricin is a protein found in castor beans that acts as an inhibitor of protein synthesis. It's a toxin involved in plant defense. A single ricin chain can inactivate thousands of ribosomes per minute. Chain B facilitates the entry of chain A into the cell.