Exact working dilution for Dot Blot, ELISA and IP needs to be determined experimentally,Antibody is purified on Protein A.Sensitivity: WB (with ECL): 1-10 ng of purified RFP or RFP fusion proteins. Primary antibody should be incubated for one hour at room temperature.
RFP(Red fluorescent protein) is a label that can be added to the protein of interest as a fusion protein and cannot be purified and detected.
For research use only, not for clinical use.