RE | N-terminal arginylation

Inquiry

Cat: PA01376

Size: 2x50 µg

Host: Rabbit

Clonality: Polyclonal

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide: H-REHKHANQHMSVC-NH2

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water to each tube

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Dot blot (Dot)

Recommended dilution

1 : 1000 (Dot)

Reactivity

Additional information

Antibody is purified using substractive purification method,MG132 or epoxomycin are recommended to use to inhibit proteasome and significantly increase signal from the arginylated proteins

Description

Arginination is the post-translational modification of an existing peptide chain by adding additional arginine. This modification not only changes the primary sequence of the protein, but also changes the surface of the protein. It is mediated by the arginine transferase ATE1. Arginination plays an important role in a variety of physiological pathways, for example, in vivo, arginination constitutes a degradation mechanism for pretreated proteins or proteolytic fragments whose n-terminus contain Asp and Glu.

For research use only, not for clinical use.