RBR1 | Retinoblastoma related protein

Inquiry

Cat: PA00322

Size: 50 µl

Host: Chicken

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Medicago sativa

Datasheet:

Product Info

Immunogen

Recombinant C-terminal fragment consisting of 236 amino acids of Arabidopsis thaliana retinoblastoma protein UniProt: Q9LKZ3, lTAIR: At3g12280

Host

Chicken

Clonality

Polyclonal

Purity

Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Format

Liquid

Storage

Store at 4°C. Upon arrival Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution

2µl (IP), 1 : 2000 (WB)

Expected | apparent MW

112 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Medicago sativa

Predicted reactivity

Camelia sinensis, Chenopodium rubrum, Cocos nucifera, Hordeum vulgare, Oryza sativa, Pisum sativum, Populus tremula, Scutelaria baicalensis, Zea mays

Not reactive in

Chlamydomonas reinhardtii

Additional information

Total IgY concentration is 30,8 mg/ml,This antibody is not suitable for immunolocalization.Methanol concentration in a transfer buffer can be considerably reduced or for a better transfer of high MW proteins (even with PVDF membrane).For immunoprecipitation start with 2 µl and titrate it depending upon your experimental conditions. Please note that you work with a total IgY fraction, which means that it will contain between 40-60 µg of total IgY (directed not only against retinoblastoma) therefore all of this IgY needs to be captured by the anti-IgY matrix.As control pre-serum for IP this product can be used, total, pre-immune IgY.

Description

Retinoblastoma protein Rb is considered to be a key regulator of G1/S phase transition by blocking S phase entry and cell growth. The plant retinoblastoma-associated (RBR) protein is homologous to the human tumor-suppressing retinoblastoma (pRb) protein. RBR protein function is controlled by phosphorylation and protein-protein interactions. Abbreviation :AtRBR

For research use only, not for clinical use.