RbcL II | Rubisco large subunit, form II

Cat: PA00631

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Alexandrium catenella, Amphidinium carterae, Chaetoceros neogracilis, Rhodobacter capsulatus, Rhodospirillum rubrum

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide conserved in known RbcL form II protein sequences

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunofluorescence (IF), Western blot (WB)

Recommended dilution

1:10 000 (IF), (WB)

Expected | apparent MW

51,7 kDa

Reactivity

Confirmed reactivity

Alexandrium catenella, Amphidinium carterae, Chaetoceros neogracilis, Rhodobacter capsulatus, Rhodospirillum rubrum

Predicted reactivity

Acidithiobacillus ferrooxidans, Dechloromonas aromatica, Gonyaulax polyedra, Leptothrix cholodnii, Magnetovibrio blakemorei, Rhodobacter sphaeroides, Thiobacillus denitrificans, Rhodopseudomonas palustrisand with a signle mismatch in one amino acid: Gallionella capsiferriformans, Mariprofundus ferrooxydans, Thioalkalicoccus limnaeus, Methanomethylovorans hollandica, Methanococcoides burtonii, Methanosaeta concili, Methanolobus tindarius, Methanohalophilus mahii, and the dinoflagellate Symbiodinium sp. (ex Stylophora pistillata)

Not reactive in

Burkholderi, Cyanobium sp.

Additional information

The antibody does not detect Form I RbcL/cbbL,Protein extraction from diatoms protocol can be found here.

Description

Rubisco(ribulose-1, 5-diphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of carbon dioxide fixation in photosynthetic organisms. Form II Rubisco is found in many photosynthetic bacteria and archaea, as well as some photosynthetic dinoflagellates. The large subunit (LSU) of Type I Rubisco is encoded by the RbcL/cbbL gene, and the large subunit of type II Rubisco is encoded by the cbbM gene.

For research use only, not for clinical use.