Vector backbone
R4pGWB401
Modifications to backbone
The sequence encoding Citrine, a dimeric acid stable yellow fluorescence protein, was ligated downstream of the attR2 gateway site using the Eco47II and SacI sites, to enable C-terminal Citrine tagging during Three-way Gateway cloning.
Selectable markers
Kanamycin (35 ug/ml)
Bacterial Resistance(s)
Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
Growth Strain(s)
ccdB Survival
Growth instructions
For selection use 100 ug/ml Spectinomycin