Quercetin 3-O-methyltransferase 1 (OMT1), Recombinant Protein
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Quercetin 3-O-methyltransferase 1 (OMT1), Recombinant Protein

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Cat: RP00865
Size: 0.02 mg (E-Coli)/ 0.02 mg (Yeast)/ 0.1 mg (E-Coli)/ 0.1 mg (Yeast)/ 0.02 mg (Baculovirus)/ 0.02 mg (Mammalian-Cell)/ 0.1 mg (Baculovirus)/ 1 mg (E-Coli)/ 1 mg (Yeast)/ 0.1 mg (Mammalian-Cell)/ 1 mg (Baculovirus)/ 0.5 mg (Mammalian-Cell)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Quercetin 3-O-methyltransferase 1 (OMT1)
Product Gene Name
OMT1 recombinant protein
Product Synonym Gene Name
OMT1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MGSTAETQLT PVQVTDDEAA LFAMQLASAS VLPMALKSAL ELDLLEIMAK NGSPMSPTEI ASKLPTKNPE APVMLDRILR LLTSYSVLTC SNRKLSGDGV ERIYGLGPVC KYLTKNEDGV SIAALCLMNQ DKVLMESWYH LKDAILDGGI PFNKAYGMSA FEYHGTDPRF NKVFNNGMSN HSTITMKKIL ETYKGFEGLT SLVDVGGGIG ATLKMIVSKY PNLKGINFDL PHVIEDAPSH PGIEHVGGDM FVSVPKGDAI FMKWICHDWS DEHCVKFLKN CYESLPEDGK VILAECILPE TPDSSLSTKQ VVHVDCIMLA HNPGGKERTE KEFEALAKAS GFKGIKVVCD AFGVNLIELL KKL
Sequence Positions
1-363, Full length protein
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
39,618 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Quercetin 3-O-methyltransferase

NCBI/Uniprot Data

NCBI Accession #
NP_200227.1
NCBI GI #
15239571
NCBI GenBank Nucleotide #
NM_124796.4
NCBI GeneID
835504
NCBI Official Full Name
O-methyltransferase 1
NCBI Official Symbol
OMT1
NCBI Official Synonym Symbols
AtCOMT; ATOMT1; caffeate O-methyltransferase 1; COMT1; K18G13.3; K18G13_3; O-methyltransferase 1; O-METHYLTRANSFERASE 1; O-methyltransferase 3; OMT3
NCBI Protein Information
O-methyltransferase 1
NCBI Summary
A caffeic acid/5-hydroxyferulic acid O-methyltransferase. Interacts with 14-4-3 proteins in yeast 2 hybrid assay. AtOMT1 (At5g54160) encodes a flavonol 3?-O-methyltransferase that is highly active towards quercetin and myricetin. The substrate specificity identifies the enzyme as flavonol 3?-methyltransferase which replaces the former annotation of the gene to encode a caffeic acid/5-hydroxyferulic acid O-methyltransferase
UniProt Gene Name
OMT1
UniProt Synonym Gene Names
COMT1; AtOMT1
UniProt Protein Name
Flavone 3'-O-methyltransferase 1
UniProt Synonym Protein Names
Acetylserotonin O-methyltransferase OMT1
UniProt Primary Accession #
Q9FK25
UniProt Secondary Accession #
O49964; Q42170
UniProt Related Accession #
Q9FK25
UniProt Comments
Methylates OH residues of flavonoid compounds. Converts quercetin into isorhamnetin. Dihydroquercetin is not a substrate. Catalyzes the methylation of monolignols, the lignin precursors. Does not contribute to the phenylpropanoid pattern of the pollen tryphine, but is probably confined to isorhamnetin glycoside biosynthesis (PubMed:10700397, PubMed:12777055, PubMed:20652169, PubMed:22258746). Involved in melatonin biosynthesis. Can function as acetylserotonin O-methyltransferase. Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine) (PubMed:25039887).

For research use only, not for clinical use.