Total vector size (bp)
11605
Modifications to backbone
bar gene cassette was inserted into multiple cloning site adjacent to T-DNA left border.
Selectable markers
Basta ; bar
Bacterial Resistance(s)
Spectinomycin, 50 μg/mL
Growth instructions
For growing Agrobacterium, 28℃ is needed and the same antibiotics selection are needed for selecting the vector. SmR gene confers resistance to spectinomycin and streptomycin in bacteria