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Pyrophosphate-energized vacuolar membrane proton pump 1 (AVP1), Recombinant Protein
Cat: RP00982
Size: 1 mg (E-Coli)/ 1 mg (Yeast)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Pyrophosphate-energized vacuolar membrane proton pump 1 (AVP1) , partial
Product Gene Name
AVP1 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MVAPALLPEL WTEILVPICA VIGIAFSLFQ WYVVSRVKLT SDLGASSSGG ANNGKNGYGD YLIEEEEGVN DQSVVAKCAE IQTAISEGAT SFLFTEYKYV GVFMIFFAAV IFVFLGSVEG FSTDNKPCTY DTTRTCKPAL ATAAFSTIAF VLGAVTSVLS GFLGMKIATY ANARTTLEAR KGVGKAFIVA FRSGAVMGFL LAASGLLVLY ITINVFKIYY GDDWEGLFEA ITGYGLGGSS MALFGRVGGG IYTKAADVGA DLVGKIERNI PEDDPRNPAV IADNVGDNVG DIAGMGSDLF GSYAEASCAA LVVASISSFG INHDFTAMCY PLLISSMGIL VCLITTLFAT DFFEIKLVKE IEPALKNQLI ISTVIMTVGI AIVSWVGLPT SFTIFNFGTQ KVVKNWQLFL CVCVGLWAGL IIGFVTEYYT SNAYSPVQDV ADSCRTGAAT NVIFGLALGY KSVIIPIFAI AISIFVSFSF AAMYGVAVAA LGMLSTIATG LAIDAYGPIS DNAGGIAEMA GMSHRIRERT DALDAAGNTT AAIGKGFAIG SAALVSLALF GAFVSRAGIH TVDVLTPKVI IGLLVGAMLP YWFSAMTMKS VGSAALKMVE EVRRQFNTIP GLMEGTAKPD YATCVKISTD ASIKEMIPPG CLVMLTPLIV GFFFGVETLS GVLAGSLVSG VQIAISASNT GGAWDNAKKY IEAGVSEHAK SLGPKGSEPH KAAVIGDTIG DPLKDTSGPS LNILIKLMAV ESLVFAPFFA THGGILFKYF
Sequence Positions
1-770
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
80,820 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Pyrophosphate-energized vacuolar membrane proton pump
NCBI/Uniprot Data
NCBI Accession #
NP_173021.1
NCBI GI #
15218279
NCBI GenBank Nucleotide #
NM_101437.5
NCBI GeneID
838138
NCBI Official Full Name
Inorganic H pyrophosphatase family protein
NCBI Official Symbol
AVP1
NCBI Official Synonym Symbols
ARABIDOPSIS THALIANA V-PPASE 3; AtAVP1; ATAVP3; AtVHP1;1; AVP-3; F7H2.3; F7H2_3; FUGU 5; FUGU5; VACUOLAR H+ - PYROPHOSPHATASE AVP-3; VHP1
NCBI Protein Information
Inorganic H pyrophosphatase family protein
NCBI Summary
Encodes a H(+)-translocating (pyrophosphate-energized) inorganic pyrophosphatase (H(+)-PPase; EC 3.6.1.1) located in the vacuolar membrane. Expression is found in all tissues examined, including meristems and floral organ primordium. Expression is particularly enhanced in pollen, and is repressed by light. Over expression and loss of function phenotypes suggest AVP1 is involved in regulation of apoplastic pH and auxin transport. The effect on auxin transport likely involves effects of extracellular pH on subcellular localization of auxin efflux carriers such as PIN1.
UniProt Gene Name
AVP1
UniProt Synonym Gene Names
AVP; AVP-3; AVP3; H(+)-PPase 1
UniProt Protein Name
Pyrophosphate-energized vacuolar membrane proton pump 1
UniProt Synonym Protein Names
Pyrophosphate-energized inorganic pyrophosphatase 1; H(+)-PPase 1; Vacuolar proton pyrophosphatase 1; Vacuolar proton pyrophosphatase 3
UniProt Primary Accession #
P31414
UniProt Secondary Accession #
O80390; Q41919; Q41920; Q56WP6; Q8RY20; Q8VZE3
UniProt Related Accession #
P31414
UniProt Comments
Contributes to the transtonoplast (from cytosol to vacuole lumen) H+-electrochemical potential difference. It establishes a proton gradient of similar and often greater magnitude than the H+-ATPase on the same membrane. In addition, facilitates auxin transport by modulating apoplastic pH and regulates auxin-mediated developmental processes. Confers tolerance to NaCl and to drought by increasing ion retention.
For research use only, not for clinical use.