Puroindoline-A (PINA), Recombinant Protein

Cat: RP16525

Size: 0.02 mg (E-Coli)/0.1 mg (E-Coli)/0.02 mg (Yeast)/0.1 mg (Yeast)/0.02 mg (Baculovirus)/1 mg (E-Coli)/0.02 mg (Mammalian-Cell)/0.1 mg (Baculovirus)/1 mg (Yeast)/0.1 mg (Mammalian-Cell)/1 mg (Baculovirus)/0.5 mg (Mammalian-Cell)

Species: Triticum aestivum (Wheat)

Datasheet:

Product Info

Full Product Name

Recombinant Triticum aestivum Puroindoline-A (PINA)

Product Gene Name

PINA recombinant protein

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

DVAGGGGAQQ CPVETKLNSC RNYLLDRCST MKDFPVTWRW WKWWKGGCQE LLGECCSRLG QMPPQCRCNI IQGSIQGDLG GIFGFQRDRA SKVIQEAKNL PPRCNQGPPC NIPGTIGY

Sequence Positions

29-146, Full length protein

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

16,387 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

Puroindoline

NCBI/Uniprot Data

NCBI Accession #

P33432.2

NCBI GI #

1709916

NCBI Official Full Name

Puroindoline-A

UniProt Gene Name

PINA

UniProt Protein Name

Puroindoline-A

UniProt Primary Accession #

P33432

UniProt Secondary Accession #

Q5BHS1; Q6J542; Q6L5P2

UniProt Comments

Acts as a membranotoxin, probably through its antibacterial and antifungal activities, contributing to the defense mechanism of the plant against predators. Forms monovalent cation-selective ion channels in membranes. Has antibacterial activity against the Gram-positive bacteria S.aureus and C.michiganensis, and the Gram-negative bacteria E.coli, P.syringae pv phaseoli, A.tumefaciens and E.carotovora subsp carotovora. Acts synergistically with PINB against bacteria. Contributes to grain texture and hardness.

For research use only, not for clinical use.