PsbX | small subunit X of PSII

Inquiry

Cat: PA00300

Size: 200 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide dervied from Arabidopsis thaliana PsbX sequence UniProt: Q9SKI3 TAIR: At2g06520

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 200 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 2500, 1 µg of chlorophyll/lane (WB)

Expected | apparent MW

4 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

Dictos, Oryza sativa, Zea mays

Not reactive in

Cyanobacteria

Additional information

PsbX is a small (4 kDa) and very hydrophobic subunit of PSII, Immunoblots from SDS-gels (especially with high loading) may show higher molecular weight signals from PsbX not fully detached from other subunits of PSII, The use of 15% SDS-PAGE gels with 2-4 M urea is recommended when working with this antibody,For an image of antibody detection in a western blot application see Garcia-Cerdan et al. (2008).

Description

Plant PsbX is a small nuclear encoded 4kda protein associated with photosystem II that is present in all species of oxygen-containing organisms.

For research use only, not for clinical use.