PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-protein)
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PsbP | 23 kDa protein of the oxygen evolving complex (OEC) of PSII (anti-protein)

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Cat: PA00295
Size: 200 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardti, Hordeum vulgare, Pinus banksiana, Spinacia oleracea, Triticum aestivum
Datasheet:

Product Info

Immunogen
native, purified 23 kDa protein from Spinacia oleracea,UniProt: P12302
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein G purified in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 200 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 2000-1 : 5000 (WB)
Expected | apparent MW
28 | 23 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Chlamydomonas reinhardti, Hordeum vulgare, Pinus banksiana, Spinacia oleracea, Triticum aestivum
Predicted reactivity
Musa acuminata, Oryza sativa, Pinus monticola, Pisum sativum, Populus balsamifera, Solanum lycopersicum
Not reactive in
Synechococcus sp. PCC 7942

Additional information

Load per well on cell extract of Pinus banksiana (Jack Pine) is 7 µg.This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).

Description

The central component of the PSII reaction generates the REDOX potential required to drive the highly oxidizing water cracking reaction. Four Mn atoms exist on the surface of the lumen, forming the catalytic site of the water decomposition reaction, which is closely related to the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) exogenous subunits of OEC. A 33 kda foreign protein is also known as mn stable protein (MSP), but recent evidence suggests that it is the C-terminal domain of PsbA (D1) protein and is involved in the assembly and stabilization of OEC.

For research use only, not for clinical use.