PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-protein)
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PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-protein)

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Cat: PA01010
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella sp. DT, Chlorella vulgaris, Echinochloa crus-galli, Eucalyptus grandis x Eucalyptus camaldulensis, Festuca arundinacea cv. Kord, Haematococcus pluvialis, Hordeum spontaneum, Hordeum vulgare, Nicotiana benthamiana, Oryza sativa, Panicum miliaceum, Picea abies, Pinus banksiana, Pisum sativum, Spinacia oleracea, Solanum tuberosum cultivar Taedong Valley, Synechococcus sp. PCC7002, sp. PCC 7942 and sp. PCC6803, Thellungiella salsuginea, Triticum aestivum, Zea mays
Datasheet:

Product Info

Immunogen
Native purified 33 kDa protein from Spinacia oleracea UniProt: P12359
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG. Protein G purified in PBS pH 7.4.
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1 : 2000-1 : 5000 (WB)
Expected | apparent MW
35 | 33 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlorella sp. DT, Chlorella vulgaris, Echinochloa crus-galli, Eucalyptus grandis x Eucalyptus camaldulensis, Festuca arundinacea cv. Kord, Haematococcus pluvialis, Hordeum spontaneum, Hordeum vulgare, Nicotiana benthamiana, Oryza sativa, Panicum miliaceum, Picea abies, Pinus banksiana, Pisum sativum, Spinacia oleracea, Solanum tuberosum cultivar Taedong Valley, Synechococcus sp. PCC7002, sp. PCC 7942 and sp. PCC6803, Thellungiella salsuginea, Triticum aestivum, Zea mays
Predicted reactivity
Glycine max, Nicotiana tabacum, Solanum lycopersicum, Populus trichocarpa, Zosteria marina
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Total IgG fraction has been purified by 40% ammonium sulpgate precipitation followed by DEAE cellulose chromatographyThis product can be sold containing ProClin if requested,This antibody can be used as a loading control for Chlamydomonas reinhardtii while it not so suitable for higher plants as accumulation of these proteins might drop to 12.5-25 % of the WT level in mutants defective for PSII core (Schult et al. 2007).

Description

The central component of the PSII reaction generates the REDOX potential required to drive the highly oxidizing water cracking reaction. Four Mn atoms exist on the surface of the lumen, forming the catalytic site of the water decomposition reaction, which is closely related to the 33 kDa (PsbO), 23 kDa (PsbP) and 17 kDa (PsbQ) exogenous subunits of OEC. A 33 kda foreign protein is also known as mn stable protein (MSP), but recent evidence suggests that it is the C-terminal domain of PsbA (D1) protein and is involved in the assembly and stabilization of OEC.

For research use only, not for clinical use.