PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide)
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PsbO | 33 kDa of the oxygen evolving complex (OEC) of PSII (anti-peptide)

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Cat: PA00289
Size: 100 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Cucumis sativus, Hordeum vulgare, Manihot esculenta, Nicotiana tabacum, Pisum sativum, Sinapsis alba, Triticum aestivum, Zea mays
Datasheet:

Product Info

Immunogen
N-terminually located peptide chosen from Arabidopsis thaliana PsbO1 and PsbO2 isoforms. UniProt: P23321 , PsbO1, TAIR: At5g66570 ; UniProt:A0A178VBH5, TAIR: At3g50820
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 100 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Recommended dilution
1 : 1000 (WB)
Expected | apparent MW
33 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Cucumis sativus, Hordeum vulgare, Manihot esculenta, Nicotiana tabacum, Pisum sativum, Sinapsis alba, Triticum aestivum, Zea mays
Predicted reactivity
Brassica oleracea, Pisum sativum, Populus tremula, Picea sitcHensis, Vitis vinifera
Not reactive in
Chlamydomonas reinhardtii, Synechococcus sp. PCC 7942

Additional information

Loading based on 50-100 ng of chlorophyll is enough to obtain good signal with this antibody,Good signal is obtained with this antibody with a load from 0,5 chlorophyll µg/well

Description

PsbO protein is a foreign subunit of the water-splitting photosystem II (PSII) complex. This protein is exposed on the lumen side of the thylakoid membrane and is highly conserved in all known oxygen-photosynthetic organisms. Other names for PsbO1 include the 33 kDa evolved oxygen system subunit of Photosystem II, the 33 kDa Evolved oxygen system subunit of OEC 33 kDa subunit, the 33 kDa thylakoid membrane protein of 33 kDa, the 33 kDa evolved oxygen system subunit of manganese stable protein 1 and the 33 KDA evolved oxygen system subunit of PsbO2 photosystem II, the 33 KDA subunit of OEC 33 kDa, and the 33 KDA subunit of PSBO2 kDa thylakoid membrane protein, manganese stable protein 2.

For research use only, not for clinical use.