PsbH | Small subunit H of PSII

This product can be sold containing ProClin if requested,LMW proteins can sometimes interfere with chlorophyll, but most chlorophyll can be removed by precipitating sample in acetone before loading on a gel.Protocol: Add acetone to final concentration of 80% ice-cold acetone. Leave 10 minutes. Spin. Rresuspend pellet in solubilisation buffer and load on a gel.

The pshh protein is originally named 10- or 9-kDa phosphorylated protein in higher plant chloroplasts. It is encoded by plastids of algae and higher plants. pshb is also found in cyanobacteria, where it has 56% amino acid agreement with the corresponding protein in Arabidopsis. The protein contains 63-90 amino acids, varies by species, and has a molecular weight of 7.0-9.9 kDa. pshb is an intrinsic membrane protein with a single transmembrane helical structure whose n-terminal region is thought to be exposed on the stromal side of the thylakoid membrane. The presence of psih already in yellows suggests that the protein may be involved in the early stages of PSII assembly. Biochemical data obtained from PSII complexes isolated from spinach suggest that psib and other PSII phosphorylated proteins may regulate the transformation of dimer and monomer PSII through phosphorylation and dephosphorylation, thereby promoting the transformation of D1 protein.