PsbD | D2 protein of PSII positive control/quantitation standard

The PsbD protein standard can be used in combination with global anti-PsbD antibodies to quantitate PsbD from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the PsbD protein. Quantitative western blot:  detailed method description, video tutorial,Concentration: after adding 225  µl of milliQ water final concentration of the standard is 0.25 pmoles/ulProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

D2 protein (PsbD) acts as a heterodimer to form PSII(Photosystem II) with D1 protein (PsbA). PsbD is homologous to D1 protein and has a slightly higher molecular weight of about 39,5 kDa. D2 protein accumulation is an important step in the assembly of the PSII reaction center complex. This product is the standard product of recombinant protein, derived from Polycytial strain pcc6803.