PsbD | D2 protein of PSII

Cat: PA00283

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Anabaena 7120, Ditylum brightwellii, Horderum vulgare, Chlamydomonas reinhardtii, Chromochloris zofingiensis, Echinola crus-galli, Emiliania huxleyi, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, picocyanobacteria, Pisum sativum, Phaseolus vulgaris, Phaeodactylum tricornutum, Trifolium pratense, Skeletonema costatum (diatom), Sinapsis alba, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Thalassiosira guillardii, Thalassiosira pseudonana, Triticale, Ulva prolifera, Zea mays

Datasheet:

Product Info

Immunogen

KLH-comjugated synthetic peptide derived from the C-terminal of known PsbD sequences including Arabidopsis thaliana P56761, Hordeum vulgare P11849, Chlamydomonas reinhardtii P06007, Synechococcus sp. PCC 7002 P20898

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Clear-native PAGE (CN-PAGE), Western blot (WB)

Recommended dilution

1: 10 000 (CN-PAGE), 1: 5000 - 1 : 50 000 (WB)

Expected | apparent MW

39,4 | 28-30 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Anabaena 7120, Ditylum brightwellii, Horderum vulgare, Chlamydomonas reinhardtii, Chromochloris zofingiensis, Echinola crus-galli, Emiliania huxleyi, Lotus corniculatus, Nicotiana tabacum, Oryza sativa, picocyanobacteria, Pisum sativum, Phaseolus vulgaris, Phaeodactylum tricornutum, Trifolium pratense, Skeletonema costatum (diatom), Sinapsis alba, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Thalassiosira guillardii, Thalassiosira pseudonana, Triticale, Ulva prolifera, Zea mays

Predicted reactivity

Aegilops tauschii, Brassica napus, Cannabis sativa, Capsicum annuum, Centrolepsis monogyna, Chromera velia, Crocosphaera watsonii, Cyanidioschyzon merolae, Fischerella sp., Galdieria sulphuraria,Glycine max, Glycine soja, Leiosporoceros dussii, Cucumis sativa, Manihot esculenta, Microcystis aeruginosa, Nannochloropsis, Panax ginseng, Petermannia cirrosa, Pinus thunbergii, Physcomitrium patens, Pinus strobus, Populus trichocarpa, Ricinus communis, Solanum tuberosum, Spinacia oleracea, Solanum lycopersicum. Triticum aestivum, Utricularia alpina, Vitis vinifera, Vitrella brassicaformis

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

The peptide used to elicit this antibody has a perfect conservation across all full-length PsbD sequences from higher plants, lower plants, cyanobacteria and unicellular algae except: minor substitutions in some Prochlorococcus & Dinoflagellate sequences, The antibody should still work against these taxa, but it has not been tested yet, This antibody does not detect PsbA protein (D1),This product can be sold containing ProClin if requested,There is a confirmed cross-reaction with TLA1 protein in Chlamydomonas reinhardtii. For samples with a very low PSII content theremight be detection problems independent of the antibody. PSII proteins can vary in level depending upon liquid culture conditions. When the cells are in a stationary phase PSII content can drop to a very low level.

Description

D2 protein (PsbD) acts as a heterodimer to form PSII(Photosystem II) with D1 protein (PsbA). PsbD is homologous to D1 protein and has a slightly higher molecular weight of about 39.5 kDa. D2 protein accumulation is an important step in the assembly of the PSII reaction center complex.

For research use only, not for clinical use.