PsbA | D1 protein of PSII positive control/quantitation standard

The PsbA protein standard can be used in combination with global anti-PsbA antibodies to quantitate PsbA from a wide range of species. Global antibodies are raised against highly conserved amino acid sequences in the PsbA protein.Quantitative western blot: detailed method description, video tutorial The goals when doing quantitative work: The sample PsbA must fall somewhere between the upper and lower standard loads.  There should be at least 3 points on the standard curve. if possible, try to make the entire range of the curve around one order of magnitude or less (as in the application example). if possible, load <5µg total sample protein. 1pmol of PsbA standard is a strong load for chemiluminescence, but may be appropriate for the less sensitive reagents, for example alkaline phosphatase.,Concentration: after adding 95 µl of sterile milliQ water final concentration of the standard is 0.25 pmoles/µlProtein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

The psbA gene has been cloned from many plants, green algae and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes the D1 protein, a core component of photosystem II. PsbA/D1 cycles rapidly under the light of all oxygen-containing photoorganisms. Tracking PsbA pools with Global PsbA antibodies can reveal the functional content of photosystem II in a wide range of samples. This is a recombinant protein standard, source: Polycystis PCC 6803.