PsbA | D1 protein of PSII, N-terminal
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PsbA | D1 protein of PSII, N-terminal

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Cat: PA00835
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Hordeum vulgare, Chlamydomonas reinhardtii, Chlorella vulgaris, Synechococcus sp. PCC7942, sp. PCC7002, Synechocystis 6803 substrain PCC-M
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from N-terminal of available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Western blot (WB)
Recommended dilution
1 : 1000-1 : 10 000 (WB)
Expected | apparent MW
38 | 28-30 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Hordeum vulgare, Chlamydomonas reinhardtii, Chlorella vulgaris, Synechococcus sp. PCC7942, sp. PCC7002, Synechocystis 6803 substrain PCC-M
Predicted reactivity
Algae (brown and red), Conifers, Cyanobacteria, Brassica napus, Diatoms, Glycine max, Manihot esculenta, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Peptide target used for antibody production comes from Helix 1 of PSII, lumenal exposed loop. Antibodies are going to recognize the target in a wide range of species.This product can be sold containing ProClin if requested.,This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel

Description

The psbA gene has been cloned from many plants, green algae and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes the D1 protein, a core component of photosystem II. PsbA/D1 cycles rapidly under the light of all oxygen-containing photoorganisms. Tracking PsbA pools with Global PsbA antibodies can reveal the functional content of photosystem II in a wide range of samples.

For research use only, not for clinical use.