PsbA | D1 protein of PSII, DE-loop
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PsbA | D1 protein of PSII, DE-loop

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Cat: PA00278
Size: 50 µl
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardii, Hordeum vulgare, Medicago truncatula, Nannochloropsis oceanica strain IMET1, Neochloris oleoabundans UTEX 1185 (chlorophyta), Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Silene vulgaris, Sinapsis alba, Spinacia oleracea, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Triticum sp.
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide, amino acids 234-242 of Arabidopsis thaliana D1 protein UniProt: P83755, TAIR:AtCg00020
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Reconstitution
For reconstitution add 50 µl of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
Blue Native PAGE (BN-PAGE), Western blot (WB)
Recommended dilution
1 : 10 000, thylakoid fraction (WB)
Expected | apparent MW
38 | 28-30 kDa

Reactivity

Confirmed reactivity
Arabidopsis thaliana, Chlamydomonas reinhardii, Hordeum vulgare, Medicago truncatula, Nannochloropsis oceanica strain IMET1, Neochloris oleoabundans UTEX 1185 (chlorophyta), Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Silene vulgaris, Sinapsis alba, Spinacia oleracea, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, Triticum sp.
Predicted reactivity
Cucumis sativus, Glycine max, Nannochloropsis sp., Oryza sativa, Populus balsamifera, Ricinus communis, Zea mays, Vitis vinifera
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Antibody will detect 23 kDa N-terminal fragment,Antibody is recognizing a 23 kDa fragment in spinach and Arabidopsis thylakoidsfor usage on total cell extracts the dilution needs to be determined experimentally

Description

The psbA gene has been cloned from many plants, green algae and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes the D1 protein, a core component of photosystem II. PsbA/D1 cycles rapidly under the light of all oxygen-containing photoorganisms. Tracking PsbA pools with Global PsbA antibodies can reveal the functional content of photosystem II in a wide range of samples. Alias :32kda thylakoid membrane protein, Photosystem II protein D1

For research use only, not for clinical use.