PsbA | D1 protein of PSII, C-terminal, FITC conjugated
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PsbA | D1 protein of PSII, C-terminal, FITC conjugated

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Cat: PA01541
Size: 40 µg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp., Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Craterostigma sp., Coscinodiscus wailesii, Cynara cardunculus var altilis, Ditylum brightwellii, Glycine max, Hordeum vulgare, Lindernia sp., Miscanthus x giganteus, Marchantia polymorpha (liverwort), Nicotiana benthamiana,Panicum miliaceum, Panax ginseng, Panicum maximum, Paulinella chromatophora (amoeba), Pinus strobus, Physcomitrium patens, Prochlorococcus sp. (surface and deep water ecotype), Synechococcus sp. PCC 7942, Spirodela polyrhiza, Symbiodinium sp., Zea mays
Datasheet:

Product Info

Immunogen
KLH-conjugated synthetic peptide derived from available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
Host
Rabbit
Clonality
Polyclonal
Purity
Immunogen affinity purified serum in PBS pH 7.4, conjugated to FITC.
Format
Liquid
Storage
Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Expected | apparent MW
38 | 28-30 kDa

Reactivity

Confirmed reactivity
Anabaena 7120, Arabidopsis thaliana, Artemisia annua, Arundo sp., Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Craterostigma sp., Coscinodiscus wailesii, Cynara cardunculus var altilis, Ditylum brightwellii, Glycine max, Hordeum vulgare, Lindernia sp., Miscanthus x giganteus, Marchantia polymorpha (liverwort), Nicotiana benthamiana,Panicum miliaceum, Panax ginseng, Panicum maximum, Paulinella chromatophora (amoeba), Pinus strobus, Physcomitrium patens, Prochlorococcus sp. (surface and deep water ecotype), Synechococcus sp. PCC 7942, Spirodela polyrhiza, Symbiodinium sp., Zea mays
Predicted reactivity
Algae (brown and red), Brassica napus, Conifers, Cyanobacteria, Dictos, Manihot esculenta, Medicago sativa, Nannochloropsis sp., Pisum sativum, Triticum aestivum, cellular [compartment marker] of thylakoid membrane.
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

PsbA | D1 protein of PSII, C-terminal, FITC conjugated,The antibody is appropriate for detecting both, 24 kDa or the 10 kDa C-terminal fragments, whichever is generated under given treatment conditions. In our analysis we have seen both, ca. 24 kDa and ca. 10 kDa fragments from different samples, depending on treatments and isolation procedures. Rabbit anti-PsbA antibody can detect more than one band of PsbA protein, e.g. precursor and mature protein as compare to the hen anti-PsbA antibodies AS01 016. This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.The antibody will bind to cross-linked proteins: D1/D2, D1/cyt b559, D1/CP43.

Description

The psbA gene has been cloned from many plants, green algae and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes the D1 protein, a core component of photosystem II. PsbA/D1 cycles rapidly under the light of all oxygen-containing photoorganisms. Tracking PsbA pools with Global PsbA antibodies can reveal the functional content of photosystem II in a wide range of samples. Alias :32kda thylakoid membrane protein, Photosystem II protein D1

For research use only, not for clinical use.