PsaD | PSI-D subunit of photosystem I

Cat: PA00788

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Dioxoniella giordanoi (red alga), Hordeum vulgare, Lactuca sativa, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Picea glauca, Pinus strobus, Oryza sativa, Physcomitrium patens, Spinacia oleracea, Synechocystis PCC 6803, Triticum aestivum, Triticale, Zea mays

Datasheet:

Product Info

Immunogen

KLH-conjugated synthetic peptide 100% conserved in all known plant PsaD sequences including Arabidopsis thaliana PSI-D1 UniProt:Q9S7H1 , TAIR: At4g02770 and PSI-D2 UniProt: Q9SA56 , TAIR At1g03130 as well as Physcomitrella patens. The conservation in Chlamydomonas reinhardtii is high (14 of 16 aminoacids are identical).

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Clear-native PAGE (CN-PAGE), Immunoprecipitation (IP), Western blot (WB)

Recommended dilution

1: 10 000 (CN-PAGE), 1 : 1000 - 1: 5 000 (WB)

Expected | apparent MW

17.9 | 20 (for Arabidopsis thaliana)

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Chlamydomonas reinhardtii, Dioxoniella giordanoi (red alga), Hordeum vulgare, Lactuca sativa, Nicotiana tabacum, Oryza sativa, Physcomitrium patens, Picea glauca, Pinus strobus, Oryza sativa, Physcomitrium patens, Spinacia oleracea, Synechocystis PCC 6803, Triticum aestivum, Triticale, Zea mays

Predicted reactivity

Alge, Dicots, Catalpa bungei, Cucumis melo, Conifers, Cyanidioschyzon merolae, Bigelowiella natans, Nannochloropsis sp. , Phaeodactylum tricornutum, Phyla dulcis, Zosteria marina

Not reactive in

Synechococcus elongatus sp. PCC 7942

Additional information

PsaD has frequently been used as a marker for intact PSI reaction centers.This product can be sold containing proclin if requested.,This antibody is a replacement for former product, anti-PsaD AS04 046 Contains 0.1% ProClin.

Description

PsaD (PSI-D) is the core subunit of photosystem I and is highly conserved in all photosynthetic organisms, including bacteria with Fe-S type reaction centers. In eukaryotes, it is encoded by one or two nuclear genes and is introduced into the chloroplast as a precursor. In the thylakoid membrane, it binds to PsaA and PsaB at the core matrix site of PSI to form the fd docking site. PsaD is also required for stable assembly of PsaC.

For research use only, not for clinical use.