PsaC | PSI-C core subunit of photosystem I (PsaC antibody + PsaC protein positive control )

Peptide target used to elicit this antibody is well conserved in all photoautotrophs except some cyanobacteria, some red algae and Cyanophora paradoxa, which contain a conserved substitution of a valine to an isoleucine. The performance of the antibodies has been confirmed against taxa containing both the valine and isoleucine variants.   For reconstitution of PsaC antibodies add 50 µl of sterile water. For reconstitution of PsaC positive control add 100 µl of sterile water.,In some species minor cross reactions with some larger proteins are seen. These may contain related iron-sulfur binding motifs. Therefore size verification of the reacting band is required. Due to the small size of the protein, care should be taken to differentiate between chemiluminescent signal from PsaC and non-specific signals from chlotophylls or lipids if pigment is retained near the bottom of the blot. Protein standard: use a load of 2 µl per well with ECL detection system and 4 µl per well with alkaline phospatase.

PsaC is a conserved chloroplast-encoded Fe-S binding protein, approximately 10 kDa, present in all known photosystem I complexes. It is located on the stromal side of the thylakoid membrane. PsaC coordinates the Fe-S clusters FA and FB through two cysteine-rich domains.