PsaC | PSI-C core subunit of photosystem I

Peptide target used to elicit this antibody is well conserved in all photoautotrophs except some cyanobacteria, some red algae and Cyanophora paradoxa, which contain a conserved substitution of a valine to an isoleucine. The performance of the antibodies has been confirmed against taxa containing both the valine and isoleucine variants. Example of a simulataneous western blot detection with RbcL, PsbA and PsaC antibodies. More information about quantitative western blot using PsaC antibody can be found here.,In some species minor cross reactions with some larger proteins are seen. These may contain related iron-sulfur binding motifs. Therefore size verification of the reacting band is required. Due to the small size of the protein, care should be taken to differentiate between chemiluminescent signal from PsaC and non-specific signals from chlotophylls or lipids if pigment is retained near the bottom of the blot. For the most optimal results use:thylakoid membranes or PSI particles,  solubilized in a SDS sample buffer (final concentrations: 63 mM Tris HCl, 10% glycerol, 2% SDS, 0.0025% bromophenol blue) with 2.5% beta-mercaptoethanol at 85C for 2 minutes. The samples are spun softly, then the supernatant loaded. This product can be sold containing ProClin if requested.

PsaC is a conserved, chloroplast-encoded, approximately 10kDa FE-S-binding protein present in all known photosystem I complexes. It is located on the stromal side of the thylakoid membrane. PsaC coordinates the Fe-S clusters FA and FB through two cysteine-rich domains.