PrxQ | Peroxiredoxin, thioredoxin reductase

Inquiry

Cat: PA00881

Size: 200 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana, Manihot esculenta, Spinacia oleracea, Zea mays

Datasheet:

Product Info

Immunogen

His-tagged full length protein (with presequence) of Arabidopsis thaliana is overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86, At3g26060

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 200 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 5000 (WB)

Expected | apparent MW

16 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana, Manihot esculenta, Spinacia oleracea, Zea mays

Predicted reactivity

Marchantia polymorpha, Populus sp. , Triticum aestivum, Oryza sativa

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Additional information

This product can be sold containing proclin if requested,In stroma fractions a weak background reaction at 28 kDa is visible, No crossreactivity in any thylakoid fractions

Description

Peroxidoredotoxins (EC=1.11.1.15) belong to a family of enzymes that are common in all biological kingdoms. prxq enzyme that acts by reducing hydroperoxides. Unlike other peroxidase enzymes, peroxidase does not have a heme group, but uses cysteine residues and REDOX active sulfhydryl groups to exert its enzymatic activity. The ability of peroxide-detoxifying toxins to hydrolyze hydroperoxides suggests that this family of proteins has a general function in oxidative defense.

For research use only, not for clinical use.