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Protein ROS1 (ROS1), partial, Recombinant Protein
Cat: RP01070
Size: 0.02 mg (E-Coli)/ 0.1 mg (E-Coli)/ 1 mg (E-Coli)/ 5x1 mg (E-Coli)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Protein ROS1 (ROS1), partial
Product Synonym Names
Protein ROS1; EC=3.2.2.-; DEMETER-like protein 1; Repressor of silencing 1
Product Gene Name
ROS1 recombinant protein
Product Synonym Gene Name
ROS1; DML1; At2g36490; F1O11.12
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MEKQRREESS FQQPPWIPQT PMKPFSPICP YTVEDQYHSS QLEERRFVGN KDMSGLDHLS FGDLLALANT ASLIFSGQTP IPTRNTEVMQ KGTEEVESLS SVSNNVAEQI LKTPEKPKRK KHRPKVRREA KPKREPKPRA PRKSVVTDGQ ESKTPKRKYV RKKVEVSKDQ DATPVESSAA VETSTRPKRL CRRVLDFEAE NGENQTNGDI REAGEMESAL QEKQLDSGNQ ELKDCLLSAP STPKRKRSQG KRKGVQPKKN GSNLEEVDIS MAQAAKRRQG PTCCDMNLSG IQYDEQCDYQ KMHWLYSPNL QQGGMRYDAI
Sequence Positions
1-320aa; Partial
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
52.3 kDa
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Protein
NCBI/Uniprot Data
NCBI Accession #
NP_181190.3
NCBI GI #
42569673
NCBI GenBank Nucleotide #
NM_129207.4
NCBI GeneID
818224
NCBI Official Full Name
protein ROS1
NCBI Official Symbol
DML1
NCBI Official Synonym Symbols
demeter-like 1; DML1; F1O11.12; F1O11_12; REPRESSOR OF SILENCING1; ROS1
NCBI Protein Information
protein ROS1
NCBI Summary
A repressor of transcriptional gene silencing. Functions by demethylating the target promoter DNA. Interacts physically with RPA2/ROR1. In the ros1 mutants, an increase in methylation is observed in a number of gene promoters. Among the loci affected by ros1, a few (RD29A and At1g76930) are affected in cytosine methylation in all sequence contexts (CpG, CpNpG or CpNpN), although many others are affected primarily in non-CpG contexts.
UniProt Gene Name
ROS1
UniProt Synonym Gene Names
DML1
UniProt Protein Name
Protein ROS1
UniProt Synonym Protein Names
DEMETER-like protein 1; Repressor of silencing 1
UniProt Entry Name
ROS1_ARATH
UniProt Primary Accession #
Q9SJQ6
UniProt Secondary Accession #
Q7Y1X6
UniProt Related Accession #
Q9SJQ6
UniProt Comments
Bifunctional DNA glycosylase/lyase, which excises 5-methylcytosine (5-meC) and 5-hydroxymethylcytosine (5-hmeC), leaving an apyrimidinic (AP) site that is subsequently incised by the lyase activity (PubMed:25240767). Generates 3'-phosphor-alpha,beta-unsaturated aldehyde (3'-PUA) as a primary 5-meC excision intermediate (PubMed:25228464). Prevents DNA hypermethylation, specifically in the promoter of otherwise silenced loci. May be involved in DNA repair through its nicking activity on methylated DNA. Binds with similar affinity to both methylated and non-methylated DNA. Highly distributive behavior on DNA substrates containing multiple 5-meC residues. Involved with Pol IV in the remodeling of the 5S rDNA chromatin via DNA methylation modifications during the first days of development post-germination. Participates in UV-B induced- and oxidative DNA damage repair (PubMed:24155752).
For research use only, not for clinical use.