Protein CLAVATA 3 (CLV3), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana Protein CLAVATA 3 (CLV3)

Product Gene Name

CLV3 recombinant protein

Product Synonym Gene Name

CLV3

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

SDLTQAHAHV QGLSNRKMMM MKMESEWVGA NGEAEKAKTK GLGLHEELRT VPSGPDPLHH HVNPPRQPRN NFQLP

Sequence Positions

22-96, Full length protein

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

10,373 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

Protein

NCBI Accession #

NP_001118398.1

NCBI GI #

186503443

NCBI GenBank Nucleotide #

NM_001124926.2

NCBI GeneID

817267

NCBI Official Full Name

CLAVATA3

NCBI Official Symbol

CLV3

NCBI Official Synonym Symbols

AtCLV3; CLAVATA3; F12K2.17; F12K2_17

NCBI Protein Information

CLAVATA3

NCBI Summary

One of the three CLAVATA genes controlling the size of the shoot apical meristem (SAM) in Arabidopsis. Belongs to a large gene family called CLE for CLAVATA3/ESR-related. Encodes a stem cell-specific protein CLV3 presumed to be a precursor of a secreted peptide hormone. The deduced ORF encodes a 96-amino acid protein with an 18-amino acid N-terminal signal peptide. The functional form of CLV3 (MCLV3) was first reported to be a posttranscriptionally modified 12-amino acid peptide, in which two of the three prolines were modified to hydroxyproline (Ito et al., Science 2006, 313:842; Kondo et al., Science 2006, 313:845). Ohyama et al. (2009) later reported that the active mature CLV3 is a 13-amino-acid arabinosylated glycopeptide (Nature Chemical Biology, 5:578). CLV3 binds the ectodomain of the CLAVATA1 (CLV1) receptor-kinase. Regulates shoot and floral meristem development. Required for CLAVATA1 receptor-like kinase assembly into a signaling complex that includes KAPP and a Rho-related protein. It restricts its own domain of expression, the central zone (CZ) of the shoot apical meristem (SAM), by preventing differentiation of peripheral zone cells, which surround the CZ, into CZ cells and restricts overall SAM size by a separate, long-range effect on cell division rate. CLE domain of CLV3 is sufficient for function. Results obtained from whole seedlings challenge the concept that the immune receptor FLS2 perceives the meristematic regulatory peptide CLV3p in mesophyll, seedlings, and SAM cells and that CLV3p contributes to SAM immunity against bacterial infection (PMID:22923673).

UniProt Gene Name

CLV3

UniProt Protein Name

Protein CLAVATA 3

UniProt Primary Accession #

Q9XF04

UniProt Secondary Accession #

Q84WX8; Q84WX9; Q9XIN2

UniProt Related Accession #

Q9XF04

UniProt Comments

Extracellular signal that regulates meristem maintenance. Acts with CLV1 as a ligand-receptor pair in a signal transduction pathway coordinating growth between adjacent meristematic regions and controlling the balance between meristem cell proliferation and differentiation.