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Protein BRASSINOSTEROID INSENSITIVE 1 (BRI1), Recombinant Protein
Cat: RP01321
Size: 1 mg (E-Coli)/ 1 mg (Yeast)
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:
Product Info
Full Product Name
Recombinant Arabidopsis thaliana Protein BRASSINOSTEROID INSENSITIVE 1 (BRI1) , partial
Product Gene Name
BRI1 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Molecular Weight
130,543 Da
Storage
Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.
Protein Family
Protein BRASSINOSTEROID INSENSITIVE
NCBI/Uniprot Data
NCBI Accession #
NP_195650.1
NCBI GI #
15235059
NCBI GenBank Nucleotide #
NM_120100.3
NCBI GeneID
830095
NCBI Official Full Name
Leucine-rich receptor-like protein kinase family protein
NCBI Official Symbol
BRI1
NCBI Official Synonym Symbols
ATBRI1; BIN1; BR INSENSITIVE 1; BRASSINOSTEROID INSENSITIVE 1; CABBAGE 2; CBB2; DWARF 2; DWF2; F23K16.30; F23K16_30
NCBI Protein Information
Leucine-rich receptor-like protein kinase family protein
NCBI Summary
Encodes a plasma membrane localized leucine-rich repeat receptor kinase involved in brassinosteroid signal transduction. BRI1 ligand is brassinolide which binds at the extracellular domain. Binding results in phosphorylation of the kinase domain which activates the BRI1 protein leading to BR responses. Residue T-1049 and either S-1044 or T-1045 were essential for kinase function in vitro and normal BRI1 signaling in planta. The structure of BRI1 ligand-binding domain has been determined at 2.5A resolution. Although BAK1 and BRI1 alone localize in the plasma membrane, when BAK1 and BRI1 are coexpressed, the heterodimer BAK1/BRI1 they form is localized in the endosome. BRI1 appears to be involved in the autonomous pathway that regulates the transition to flowering, primarily through its effects on FLC expression levels, as uncovered by double mutant analyses. This most likely occurs as a result of BRI1-dependent effects on histone acetylation, but not histone triMeH3K4 methylation, at the FLC locus.
UniProt Gene Name
BRI1
UniProt Synonym Gene Names
AtBRI1
UniProt Protein Name
Protein BRASSINOSTEROID INSENSITIVE 1
UniProt Synonym Protein Names
Brassinosteroid LRR receptor kinase
UniProt Primary Accession #
O22476
UniProt Secondary Accession #
C0LGS4
UniProt Related Accession #
O22476
UniProt Comments
Receptor with a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Regulates, in response to brassinosteroid binding, a signaling cascade involved in plant development, including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Binds brassinolide, and less effectively castasterone, but not 2,3,22,23-O-tetramethylbrassinolide or ecdysone. May be involved in a feedback regulation of brassinosteroid biosynthesis. Phosphorylates BRI1-associated receptor kinase 1 (BAK1), Transthyretin-Like protein (TTL) and SERK1 on 'Ser-299' and 'Thr-462' in vitro. May have a guanylyl cyclase activity (PubMed:10557222, PubMed:10938344, PubMed:17138891, PubMed:17520012, PubMed:18694562, PubMed:19124768). Phosphorylates BSK1, BSK2 and BSK3 in vitro (PubMed:18653891). Phosphorylates BSK1, BSK3, BSK5, BSK6, BSK8 AND BSK11 in vitro (PubMed:23496207).
For research use only, not for clinical use.