Protein argonaute 1 (AGO1), Recombinant Protein

Full Product Name

Recombinant Arabidopsis thaliana Protein argonaute 1 (AGO1) , partial

Product Gene Name

AGO1 recombinant protein

Purity

Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)

Sequence

QFVCDLLNRD ISSRPLSDAD RVKIKKALRG VKVEVTHRGN MRRKYRISGL TAVATRELTF PVDERNTQKS VVEYFHETYG FRIQHTQLPC LQVGNSNRPN YLPMEVCKIV E

Sequence Positions

391-501, Partial, provide the PAZ domain.

Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Molecular Weight

116,460 Da

Storage

Store at -20℃. For long-term storage, store at -20℃ or -80℃. Store working aliquots at 4℃ for up to one week. Repeated freezing and thawing is not recommended.

Protein Family

Protein argonaute

NCBI Accession #

NP_001185169.1

NCBI GI #

334183151

NCBI GenBank Nucleotide #

NM_001198240.1

NCBI GeneID

841262

NCBI Official Full Name

Stabilizer of iron transporter SufD / Polynucleotidyl transferase

NCBI Official Symbol

AGO1

NCBI Official Synonym Symbols

ARGONAUTE 1; AtAGO1; ICU9; T1N15.2; T1N15_2

NCBI Protein Information

Stabilizer of iron transporter SufD / Polynucleotidyl transferase

NCBI Summary

Encodes an RNA Slicer that selectively recruits microRNAs and siRNAs. There is currently no evidence that AGO1 Slicer is in a high molecular weight RNA-induced silencing complex (RISC). Mutants are defective in post-transcriptional gene silencing and have pleiotropic developmental and morphological defects. Through its action on the regulation of ARF17 expression, the protein regulates genes involved at the cross talk between auxin and light signaling during adventitious root development. AGO1 seems to be targeted for degradation by silencing suppressor F-box-containing proteins from Turnip yellow virus and Cucurbit aphid-borne yellow virus.

UniProt Gene Name

AGO1

UniProt Protein Name

Protein argonaute 1

UniProt Primary Accession #

O04379

UniProt Secondary Accession #

Q0WSQ4; Q3ECU7; Q9LP83

UniProt Related Accession #

O04379

UniProt Comments

Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Requires DRB1 for directional loading of the small RNA duplex (guide stand and passenger strand) onto RISC for passenger strand degradation. Unlike animal RISC that associates in high molecular weight complex, Arabidopsis RISC is probably composed only of the AGO1 protein and associated RNA without any other proteins. Associates mainly with miRNAs of 21 nucleotide in length and preferentially recruits small RNAs with a 5' terminal uridine. Associates with 22 nucleotide miRNAs to trigger RDR6-dependent secondary siRNAs biogenesis. This pathway amplifies silencing by using the target RNA as substrate to generate secondary siRNAs. Binds to miR168 which targets its own mRNA for repression, establishing a homeostatic regulatory loop. Involved in antiviral RNA silencing by contributing to viral RNA clearance. Is capable of targeting viral RNAs with more compact structures than AGO7 which favors less structured RNA targets. May not associate with 24 nucleotide siRNAs involved in chromatin silencing. Essential for multiple processes in development. Essential for proper development of leaves and floral organs, and formation of axillary meristems. Like AGO10, required for stem cell function and organ polarity.