Probable steroid reductase DET2 (DET2), Recombinant Protein
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Probable steroid reductase DET2 (DET2), Recombinant Protein

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Cat: RP01512
Size: 0.02 mg/ 0.1 mg/ 5x0.1 mg
Species: Arabidopsis thaliana (Mouse-ear cress)
Datasheet:

Product Info

Full Product Name
Recombinant Arabidopsis thaliana Probable steroid reductase DET2 (DET2)
Product Gene Name
DET2 recombinant protein
Purity
Greater or equal to 85% purity as determined by SDS-PAGE. (lot specific)
Sequence
MEEIADKTFF RYCLLTLIFA GPPTAVLLKF LQAPYGKHNR TGWGPTVSPP IAWFVMESPT LWLTLLLFPF GRHALNPKSL LLFSPYLIHY FHRTIIYPLR LFRSSFPAGK NGFPITIAAL AFTFNLLNGY IQARWVSHYK DDYEDGNWFW WRFVIGMVVF ITGMYINITS DRTLVRLKKE NRGGYVIPRG GWFELVSCPN YFGEAIEWLG WAVMTWSWAG IGFFLYTCSN LFPRARASHK WYIAKFKEEY PKTRKAVIPF VY
Sequence Positions
1-262aa; full length protein
Format
Liquid containing glycerol
Host
Cell Free Expression
Molecular Weight
30,635 Da
Storage
Store at -20℃, for extended storage, conserve at -20℃ or -80℃.Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Protein Family
Steroid 5-alpha-reductase

NCBI/Uniprot Data

NCBI Accession #
NP_181340.1
NCBI GI #
15224430
NCBI GenBank Nucleotide #
NM_129361.4
NCBI GeneID
818383
NCBI Official Full Name
probable steroid reductase DET2
NCBI Official Symbol
DET2
NCBI Official Synonym Symbols
ATDET2; DE-ETIOLATED 2; DET2; DWARF 6; DWF6; STEROID REDUCTASE DET2; T8P21.4; T8P21_4
NCBI Protein Information
probable steroid reductase DET2
NCBI Summary
Similar to mammalian steroid-5-alpha-reductase. Involved in the brassinolide biosynthetic pathway.
UniProt Gene Name
DET2
UniProt Synonym Gene Names
CRO1; AtDET2
UniProt Protein Name
Steroid 5-alpha-reductase DET2
UniProt Synonym Protein Names
Protein COMPACT ROSETTE 1; Protein DEETIOLATED 2
UniProt Entry Name
DET2_ARATH
UniProt Primary Accession #
Q38944
UniProt Secondary Accession #
Q9SH83
UniProt Related Accession #
Q38944
UniProt Comments
Involved in a reduction step in the biosynthesis of the plant steroid, brassinolide; acts at the second step in brassinolide biosynthesis in the 5alpha-reduction of (24R)- 24-methylcholest-4-en-3-one, which is further modified to form campestanol. Can use progesterone, testosterone, androstenedione and campestenone as substrate.

For research use only, not for clinical use.