Vector backbone
pCAMBIA1300
Modifications to backbone
1. Removed Bsa I sites.
2. Inserted the U3-gRNA-35S-Cas9 cassette from pRGE31.
Selectable markers
Hygromycin
Bacterial Resistance(s)
Kanamycin, 50 μg/mL
Growth instructions
For agrobacterium strains like EHA105, growth temperature is 28℃.