PR-2 | GLU I | Class I beta-1,3-glucanase

Cat: PA00872

Size: 2 mg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Fragaria vesca, Lactuca sativa, Nicotiana benthamiana, Nicotiana clevilandii, Nicotiana glutinosa, Nicotiana tabacum, Phalenopsis Sogo Yukidian cultivar V3, Populus sp., Solanum lycopersicum, Solanum tuberosum, Vitis vinifera

Datasheet:

Product Info

Immunogen

Purified tobacco class I, basic ß-1,3-glucanase. Purified GLU I consists of a mixture of closely related polypeptides encoded by a family of GLU I genes comprising GLA B5APL3 derived from the sylvestris ancestor of tobacco, GLB P27666 derived from the tomentosiformis ancestor of tobacco and homeologous recombinants (Sperisen et al., 1991). Mature GLU I is processed from a pre-pro-polypeptide (Shinshi et al., 1988).

Host

Rabbit

Clonality

Polyclonal

Purity

Total IgG in PBS pH 7.4. (without Ca++).

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Immunolocalization (IL), Western blot (WB)

Recommended dilution

8 µg/ml (WB)

Expected | apparent MW

37 | 33 kDa

Reactivity

Confirmed reactivity

Fragaria vesca, Lactuca sativa, Nicotiana benthamiana, Nicotiana clevilandii, Nicotiana glutinosa, Nicotiana tabacum, Phalenopsis Sogo Yukidian cultivar V3, Populus sp., Solanum lycopersicum, Solanum tuberosum, Vitis vinifera

Predicted reactivity

Dicots, Oryza sativa, Prunus persica

Not reactive in

Arabidopsis thaliana

Additional information

For more details on immunolocalization, please referr to Keefe et al (1990). Plant 182: 43-51. This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.,Important note: for blocking 5 % skim milk in PBS without Ca++ should be used. This antibody is purified by affinity chromarography on Portein G.

Description

Pathogenicity associated proteins (PR) are induced when a plant is infected by a microbial pathogen. The combination of glucanase I and chitinase I is a potent inhibitor of fungal growth in vitro, but its exact mechanism is unknown. Glucanase I and chitinase I help fight fungal infections and are currently used as markers of innate immunity, particularly in the pathogenesis of the ethylene/jasmonic acid signaling pathway. Another name for this protein is alkali-1, 3-glucanase

For research use only, not for clinical use.