PPDK | Pyruvate orthophosphate dikinase

Cat: PA01050

Size: 20 µg

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Arabidopsis thaliana,Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus, Zea mays

Datasheet:

Product Info

Immunogen

Purified recomibinant enzyme consisting of residues 72-947 of Zea mays, UniProt: P11155 Peptide used to elicit this antibody is conserved in both isoforms of PPDK in rice: PPDK1 and PPDK2.

Host

Rabbit

Clonality

Polyclonal

Purity

Immunogen affinity purified serum in PBS pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 200 µl of sterile water in 40% glycerol to a final protein concentration of 100 ng/µl

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 25 000 (WB)

Expected | apparent MW

102 | 95 kDa

Reactivity

Confirmed reactivity

Arabidopsis thaliana,Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus, Zea mays

Predicted reactivity

Hordeum vulagre, Kalanchoe fedtschenkoi

Not reactive in

Cucumis sativus

Additional information

PPDK levels inr C3 plants like Arabidopsis thaliana and Hordeum vulgare are very low and PPDK protein is very dilute in most tissues of C3 plants. To perform detection in C3 plants leaf proteins needs to be concentrated before western blot, Chastain et al. (2002).

Description

PPDK(pyruvate, phosphodikinase 1) is involved in the formation of phosphoenolpyruvate and is activated by photoinduced dephosphorylation. Dark-induced inhibition of phosphorylation. PPDK is a low abundance enzyme in C3 plants and is a key enzyme in C4 photosynthesis.

For research use only, not for clinical use.