Total vector size (bp)
11130
Vector backbone
pMpGWB102
Modifications to backbone
These PCR-amplified DNA fragments of Citrine-PTS1 were cloned into a pDONR207 plasmid by BP reaction (Invitrogen). The resulting plasmids were mixed with pMpGWB102 vector, and LR reaction (Invitrogen) was performed to generate pMpGWB102-Citrine-PTS1.
Bacterial Resistance(s)
Spectinomycin, 50 μg/mL