Vector backbone
pMpGWB303
Modifications to backbone
Atco_Cas9 CDS and Pea3A terminator were introduced by LR reaction, an then the attR1-attR2 destination cassette was cloned into the Aor51HI site.
Selectable markers
Chlorsulfuron
Bacterial Resistance(s)
Chloramphenicol and Spectinomycin, 25 & 50 μg/mL
Growth Strain(s)
ccdB Survival