PLGG1 | Plastidal glycolate/glycerate translocator 1, (chloroplastic)

Inquiry

Cat: PA01374

Size: 50 µl

Host: Rabbit

Clonality: Polyclonal

Confirmed reactivity: Nicotiana tabacum

Datasheet:

Product Info

Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana UniProt: Q9FVQ4

Host

Rabbit

Clonality

Polyclonal

Purity

Serum

Format

Lyophilized

Reconstitution

For reconstitution add 50 µl, of sterile water

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Application

Western blot (WB)

Recommended dilution

1 : 1000 (WB)

Expected | apparent MW

54 kDa

Reactivity

Confirmed reactivity

Nicotiana tabacum

Predicted reactivity

Arabidopsis thaliana, Noccaea caerulescens

Not reactive in

Vigna radiata

Additional information

Experimental contitions: 5 µg of total protein extracted freshly from 3-4 weeks old plant leaves with a blender at 4 °C in 300 mM Sorbitol, 50 mM HEPES, 5mM MgCl2. Separated on 10 % SDS-PAGE and blotted 1h to PVDF, semi-dry. Blot is blocked with 6 % milk for 1h 4°C with agitation. Blot is incubated in the primary antibody at a dilution of 1: 1 000 ON at 4°C with agitation. According to South et. al (2019).

Description

PLGG1 (Plastidal glycolate/glycerate translocator) is a glycolate/glycerate translocator required for photorespiration. Protein nicknames :AtLrgB, LrgB, PLGG

For research use only, not for clinical use.