PLD | phospholipase D, Biotin conjugated
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PLD | phospholipase D, Biotin conjugated

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Cat: PA01215
Size: 10 mg
Host: Rabbit
Clonality: Polyclonal
Confirmed reactivity: Brassica oleracea
Datasheet:

Product Info

Immunogen
Phospholipase D isolated and purified from Brassica oleracea
Host
Rabbit
Clonality
Polyclonal
Purity
Purified IgG in PBS.
Format
Lyophilized
Reconstitution
For reconstitution add 0,5 ml of sterile destilled water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Application
ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Recommended dilution
1 : 1000-1 : 20 000 (ELISA), (IF), (IHC), (WB)
Expected | apparent MW
92 kDa

Reactivity

Confirmed reactivity
Brassica oleracea
Predicted reactivity
Arabidopsis thaliana, Cucumis melo, Ricinus communis, Populus balsamifera
Not reactive in
No confirmed exceptions from predicted reactivity are currently known

Additional information

Antibody is labelled with biotin using N-hydroxysuccinimidobiotin, Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition,Biotin/IgG protein molar ration is approximately 6,2, No foreign proteins are added

Description

Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular processes, including plant hormone action, membrane degradation, senescence, etc. Alias: Phosphatidylcholine phospholipid hydrolase.

For research use only, not for clinical use.