Plasma Membrane Protein Isolation and Cell Fractionation Kit

The plasma membrane protein separation and cell Separation kit represents the next generation of plasma membrane (PM) separation and cell separation products, providing unmatched convenience and consistency by eliminating the uncontrolled changes associated with traditional homogenization and density gradient centrifugation, including two-phase separation.

When the high-speed centrifugal force acts, the cells/tissues are first sensitized by buffer A and then pass through a proprietary filter in a zigzag fashion, resulting in a cell lysate containing ruptured cell membranes and intact nuclei. As a result, nuclear contamination is virtually eliminated. PM is further separated from the cell lysate (a mixture of coarse membrane, intact nucleus, cytoplasmic proteins, and organelles), followed by differential and density centrifugation with a conventional table microcentrifuge. Five different cell components (total membrane, PM, cytoplasm, nucleus, and organelle) can be obtained. The whole process can be completed in 45 minutes.

Buffer A 25 ml
Buffer B 10 ml
Protein Extraction Filter Cartridges 50 units
Collection Tubes with Caps 50 units
Plastic Rods 2 units
Tissue Dissociation Beads 10 grams