Plant Transmembrane Protein Extraction Kit

This product is a high yield transmembrane protein extraction kit based on chemical method. The Plant Transmembrane Protein Extraction Kit can extract transmembrane proteins from various plant samples, and can be used for crude preparation of purified proteins and transmembrane protein preparation. The extraction process is simple and convenient. The kit contains a protease inhibitor mixture and a phosphatase inhibitor mixture, which prevents the degradation of proteins by proteases and provides for the extraction of high-quality proteins.
The proteins extracted in this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelate chromatography. The proteins extracted in this kit can be used in downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel blocking assay, enzyme activity assay, and so on.
The protein samples extracted from this kit contain high concentration of salt and cannot be used directly in 2D electrophoresis.

Reduce the time of protein extraction to 1 hour.
Contains protein stabiliser for stable extracted protein.
Low background interference in UV detection of protein concentration.
Protease inhibitors inhibit protein degradation and the protease inhibitor formulation is optimised. The Protease Inhibitor Blend contains six individual protease inhibitors; each inhibitor specifically inhibits one or more protease activities. The mixture's optimised composition allows it to inhibit almost all essential protease activities, including serine proteases, cysteine acid proteases, aspartate proteases, alanyl-aminopeptidases, and others.

Plant transmembrane protein extract A 25mL/50mL
Plant transmembrane protein extract B 250μL/500μL
Transmembrane protein lysis solution C 10mL/20mL
Protease inhibitor mixture 100μL/200μL