Plant Total Protein Quantification Service
Protein quantification is fundamental to systems biology and proteomics research. Quantitative analysis of plant proteins is a fundamental step in protein functional determination. We quantify total plant proteins based on the characteristic that the chromogenic groups of proteins or their binding with chromogenic agents produce specific ultraviolet absorption wavelengths. Accurate quantification of plant proteins can further elucidate the mechanisms of action in plant growth, development, metabolism, and disease resistance.
CD BioSciences provides quantitative analysis of total plant proteins based on the physical, chemical, and staining properties of the proteins. Our services help customers gain a deeper understanding of proteins in biological experiments.
Service Content
Quantification of plant total protein based on the 280nm UV absorption (range, 0.1-100mg/ml)
| Method | Description | Sample Requirements |
| UV absorption value at 280 nm | We can quantify proteins containing aromatic amino acids with special UV absorption spectra at 280nm. | The sample is pure and free of impurities. |
Quantification of plant total protein based on the Diquinolinic acid (BCA) (range, 5-2000μg/ml)
| Method | Description | Sample Requirements |
| BCA | We can quantify most plant proteins using the BCA method based on biuret reaction. This method has a wide application range and low variability. | The protein does not contain chemical modification residues and samples do not contain reducing sugars. |
Quantification of plant total proteinbased on the Bradford or Coomassie leucocyanin (range, 20-2000μg/ml)
| Method | Description | Sample Requirements |
| Bradford or Coomassie leucocyanin | We offer protein quantification methods that are compatible with reducing agents in solution for the quantification of high molecular weight (MW) proteins. | Proteins contain arginine, tryptophan, tyrosine, histidine, and phenylalanine residues. |
Quantification of plant total protein based on Lowry (range, 10-1000μg/ml)
| Method | Description | Sample Requirements |
| Lowry | We provide the Lorry method to represent the total protein concentration by the ratio of sample solution color to protein concentration. It is also suitable for the determination of tyrosine and tryptophan | High protein concentration.
The sample does not contain phenols or citric acid. |
Quantification of plant total protein based on CBQCA (range, 1000ng-15000μg/ml)
| Method | Description | Sample Requirements |
| CBQCA | We offer a CBQCA method that is insensitive to certain interfering substances such as lipids. The method can be used to quantify peptides and proteins adsorbed or encapsulated on surfaces. | Protein contains primary amines. |
Application Fields
- Food inspection for protein content compliance.
- Plant disease diagnosis.
- Evaluating and monitoring the productivity of cell lines.
- Screening of optimal protein candidates for the development of therapeutic antibodies.
- The basis of protein research experiments, such as protein chromatographic separation, protein electrophoresis analysis, total protein quantification of cell cleavage and release, and labeling of protein molecules.
CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.
For research use only, not for clinical use.