Plant Total Protein Extraction Kit (Denatured)

The kit is applied to the research of mucilaginous plant tissues or organs such as seedlings of cassava and rapeseed, leaves of potato, apple and banana, olive leaves, and fruits of tomato, avocado and banana.Carpentier et al, Saravanan and Rose compared the extraction of proteins from mucilaginous tissues by TCA/acetone precipitation and phenol extraction, and found that both methods are effective but that the The phenol extraction method is more effective in removing interfering substances and yielding high quality gels with a light background and less vertical drag. Both methods reduce protein degradation due to intrinsic protein hydrolysis activity during sample preparation. Also, the phenol extraction method yields more glycoproteins.

Phenol extraction has a high degree of cleaning power. It is able to act as a separating agent and reduces the interaction between proteins and other molecules. The main disadvantage of the phenol extraction method is that it is too time consuming (at least 6h), in addition both phenol and methanol are toxic. All operations are carried out in a biological safety cabinet.