Plant-Specific Protein Quantification Service

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Plant-Specific Protein Quantification Service

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Plant-specific proteins include functional proteins, enzymes, post-translational modifications (PTM), and low-specificity target proteins. Accurate and sensitive quantification of specific proteins is the key to most protein assays. Our methods for protein-specific quantification include enzyme-linked immunosorbent assay (ELISA), western blot, and mass spectrometry.

CD BioSciences has advanced instruments. We use fluorometers, spectrometers, and enzyme markers to quantify proteins fluorometrically or colorimetrically. Our services can be used for protein expression levels analysis, biomarker concentrations assessment, and protein-protein interactions detection.

Service Content

Quantification of plant-specific protein based on mass spectrometry

We offer mass spectrometry-based quantification of plant-specific proteins, including MALDI-TOF and ESI-MS.

Method Description Sample Requirements
MALDI-TOF MALDI is suitable for the quantification of proteinaceous samples for which ionization produces fragmentation. Protein purity > 90%; protein content > 5μg, concentration > 0.1μg/μl.
Bidirectional gel electrophoresis spots of staining or silver staining are visible.
The SDS-PAGE strip is a single protein, and the staining or silver-staining bands are visible.
ESI-MS ESI-MS can analyze protein samples that are strongly polar, difficult to volatilize, or thermally unstable. Protein content > 5μg, concentration > 0.1μg/μl. Buffer does not contain detergent NP40, Triton X-100, etc.
Cow staining or silver staining bands of SDS-PAGE adhesive strips are visible.

Quantification of plant-specific protein based on ELISA

Quantification of plant-specific protein based on ELISA

We provide enzyme-linked immunosorbent assays (ELISA) that can easily quantify a large number of samples for qualitative and quantitative analysis.

Method Description Sample Requirements
Direct ELISA Direct ELISA requires the preparation of various coupled antibodies and is suitable for the quantification of antigens. Plant tissue sample > 10g.
Antigen or antibody concentration > 1-20μg/ml, volume > 200μl.
Indirect ELISA Indirect ELISA is commonly used to quantify total antibodies.
Sandwich ELISA Sandwich Elisa is suitable for the quantification of antigens or antibodies in complex samples.

Quantification of plant-specific protein based on nanoparticles and nanopores

Method Description Sample Requirements
Nanoparticle Sensors Nanoparticles are chemically cross-linked to proteins, allowing the detection and quantification of specific proteins, such as antibodies, peptides, and ligands, based on absorption spectra. Plant tissue sample > 5g.
Protein concentration > 1-20μg/ml,  volume > 200μl.

Quantification of plant-specific protein based on western blot

Method Description Sample Type
Western Blot Western blotting can only be used for semi-quantitative or relative quantitative analysis. SDS-PAGE protein bands, 2D protein spots, pull-down, and Co-IP samples.

Application Fields

  • Diagnosis of specific plant diseases.
  • Measurement of specific post-translational modifications (PTM).
  • Measure functional proteins or enzymes.
  • Determine whether the protein can be labeled with biotin or reporter enzymes.

CD BioSciences is a biotechnology company focused on plant protein research, providing a variety of related services and products for environmental and energy solutions. If you are interested in our services, please contact us for more details.

Reference

  1. Kong J, et al. (2016). Quantifying Nanomolar Protein Concentrations Using Designed DNA Carriers and Solid-State Nanopores. Nano Lett. 16(6):3557-3562.

For research use only, not for clinical use.